Twentieth-century biochemistry deconvolved Nature’s web of metabolism and articulated enzyme active-site architecture, and with this knowledge, we have advanced industry, medicine, and biology. However, a richer depth of understanding is needed to meet growing synthetic demands, fulfill the promises of precision health, and preserve our environment. My work focuses on implementing the needed technology to provide this deeper understanding. I am developing a microfluidic-based high-throughput platform to make quantitative measurements of enzyme function at an unprecedented scale. With this technology, I aim to 1) understand the molecular details of how enzymes achieve catalytic prowess, 2) dissect molecular bases for enzyme regulation, and 3) generate foundational datasets to train computational algorithms predicting mutational effects on enzyme function. Starting with a pioneering study of phosphatases, enzymes central to normal cell function and human disease, this work has the potential to revolutionize how enzymes are studied and harnessed to transform health and industry.