Adi Mukund

Gene expression in humans is regulated in part by numerous chromatin regulators (CRs) that use effector domains to activate and repress genes in a coordinated manner. Understanding how CRs work together to affect gene expression is critical to understanding gene regulation in human cells. In this project I will combine recruitment assays to measure transcription-regulatory function with high-throughput perturbation technologies and computational modeling. I will build a quantitative model of how different human protein domains combine to affect transcription; this will deepen our understanding of chromatin biology and expand the range of tools we can use to build chromatin-based gene therapies.